Journal: Advances in Wound Care

Article Title: A Small Chimeric Fibronectin Fragment Accelerates Dermal Wound Repair in Diabetic Mice

doi: 10.1089/wound.2015.0666

Figure Lengend Snippet: FNIII1HRGD supports MSC adhesion and proliferation. Tissue culture plates were precoated with increasing concentrations of FNIII1HRGD (50–400 nM). MSCs were seeded (1 × 104 cells/cm2) onto protein-coated plates. (A) Phase contrast microscopy images were taken at 72 h postseeding. Images represent one of three experiments performed. Scale bar, 50 μm. (B) Cell number was determined at 1, 4, and 7 days postseeding using crystal violet, as described in the Materials and Methods section. Data are presented as mean absorbance ± SEM of three experiments performed in triplicate. *Significantly different from FNIII1HRGD at corresponding time point, p < 0.05 (ANOVA). MSC, mesenchymal stem cell.

Article Snippet: 21 Human mesenchymal stem cells (MSCs) and MSC Growth Medium were from Lonza.

Techniques: Microscopy

Journal: Bioactive Materials

Article Title: In situ self-assembled organoid for osteochondral tissue regeneration with dual functional units

doi: 10.1016/j.bioactmat.2023.04.002

Figure Lengend Snippet: In vitro chondrogenic and osteogenic pre-differentiation induction of MSC-seeded microcryogels. (A) Schematic of the chondrogenic induction process. (B) Toluidine blue and Alcian blue staining of control microcryogels and CH-Microcryogels after 7 d of chondrogenic induction. Relative gene expression of (C) COL2, (D) SOX9, and (E) ACAN. (F) Schematic of the osteogenic induction process. (G) Alizarin red staining of control microcryogels and OS-Microcryogels after 7 d of osteogenic induction. (H) Quantitative detection of ALP in MSCs after 7 d of osteogenic induction on control microcryogels and OS-Microcryogels. (I) Quantitative detection of ALP in MSCs on 2D culture plates. Relative gene expression of (J) RUNX2, (K) OCN, (L) COL1, and (M) ALP. (N) Atomic force microscopy (AFM) of microcryogels after 7 d of chondrogenic and osteogenic induction. F-actin and DAPI staining of MSCs cultured in microcryogels after 7 d of (O) chondrogenic induction and (P) osteogenic induction. (*p < 0.05, **p < 0.01, ***p < 0.005, n. s. represents no significant difference, n = 3).

Article Snippet: Human umbilical cord MSCs were acquired from EUBIO Technologies and cultured in MSC growth medium (BioWit Technologies), as previously described [ 22 ].

Techniques: In Vitro, Staining, Control, Gene Expression, Microscopy, Cell Culture